Abstract Background Numerous multiplex-PCR assays are now available in routine diagnostics but their clinical value is controversial if a clear association between clinical symptoms and the detection of a particular pathogen is missing.The objective of this work was to evaluate a multiplex-PCR assay for the diagnosis of traveller’s diarrhoea (TD) in a case-control study and to assess the concordance with the BioFire® FilmArray® Gastrointestinal Panel.Methods Stool samples from cases (n = 61) and controls (n = 30) were collected during travel and analysed by the GI-EB Screening assay (Seegene) in a case-control study.
The concordance with the BioFire® FilmArray® Gastrointestinal Panel was expressed as the proportion of participants in which both tests agreed in the category “detected” and “not detected”.
The effect of interurban movements on the spatial distribution of population
.Results None of the test-target organisms (Campylobacter spp., Clostridioides difficile toxin A/B, Salmonella spp.
, Shigella spp.
Characterizing the epidemiology of Mycoplasma pneumoniae infections in China in 2022–2024: a nationwide cross-sectional study of over 1.6 million cases
./enteroinvasive Escherichia coli, E.coli O157, Shiga toxin-producing E.
coli, Yersinia enterocolitica) was significantly associated with TD GI-EB Screening assay.The GI-EB Screening assay had an agreement with the BioFire® FilmArray® of 86.8–100%.
Conclusion The selection of test-target organisms included in the GI-EB Screening assay appears inappropriate for the diagnostic work-up of TD as none of the detected pathogens was associated with TD.The GI-EB Screening assay had a good concordance with BioFire® FilmArray®